Cryopreservation is a medicinal process of preserving biological function by freezing and storing material in the lab under 80 degrees Celsius. To be precise, it is the process of keeping the embryos, sperms and tissues from ovaries in extremely low temperature in the lab to use it in the future. This is the best cost-saving method for couples and reduces their discomfort. Professional fertility centre like Venus IVF in Nepal performs this task in two steps. Firstly, the sample is collected from a female or male partner and is later stored in the tube. The tube is later stored in the lab below zero degree Celsius added with a special solution to avoid getting frozen.
More and more fertility specialist recommends couples for this procedure because it can be later used without going through the extraction process again. Furthermore, woman and male don’t have to undergo the physical discomfort again. There are three types of Cryopreservation:
- Sperm Freezing
- Egg and Ovarian Tissue Freezing
- Embryo Freezing
There are multiple challenges fertility specialists face during the Cryopreservation. Some of these are;
- Helping cells to service in lowest possible temperature and thawing back to the body
- Intracellular ice formation is yet another challenge because it needs to be controlled to keep the cell membrane intact.
- To overcome these challenges, specialists in Venus IVF use modern methods and steps to get the right result on its use.
Three Important Protocols:
In Cryopreservation process, there are three important protocols and the important one is the Freezing Protocols. Under this, freezing of tissue depends on the type of tissues are preserved before any of the below-mentioned protocols. Every tissue carries different composition and therefore the specialist will work on protocols accordingly.
- Slow Protocol:
Under this protocol, the temperature is gradually reduced so that reproductive tissues get cool down gradually. Once the desired temperature is reduced after decreasing it, the acceleration rate is reduced to -0.3 degree Celsius.
2. Rapid Protocol:
Under this process, the tissues are directly plunged into liquid nitrogen and the temperature falls to 0-196 degree Celsius. Later high concentration of cryopreservation is used for avoiding crystallization.
3. Thawing Protocols:
This is a complex process to thaw the cryopreserved tissues and bringing into the room temperature. The cryopreserved tissues are placed in water baths until the room temperature rises.
The risk in Cryopreservation Method:
There is a number of risks involved in Cryopreservation, in terms of loss or damage of reproductive tissues. Many small fertility centres with non-trained professionals engage in damage of patient material. Some other risks involved are-
- Solution Effects;
The ice crystals growing in freezing water cause them to turn concentrated. High concentration can prove a risk to the health.
Migration of water and causing of extracellular ice formation leads to cellular dehydration.
Types of Cryopreservation:
There are different types of cryopreservation method performed in the fertility centre. These are;
- Embryos Cryopreservation:
This is for the storage of embryo. Under this type of cryopreservation, pregnancies have been reported. This method is performed when vitro fertilization has resulted in more embryos than the required ones.
- Ovarian Tissues;
Ovarian Cryopreservation is done when the woman wants to preserve reproductive function due to the complication of cancer therapy. At a fertility centre, the method is performed under perfection to give a positive result.
- Oocytes Cryopreservation:
This is the new technology conducted by the fertility centre in which woman’s eggs are extracted, frozen and stored. Later, when the woman is ready for pregnancy, eggs are thawed, fertilized and transferred through the process into the uterus. For this process, Oocyte vitrification is quite important to preserve the fertility in young patients undergoing IVF treatment.
If you are looking forward to Cryopreservation method for pregnancy, then connect with Venus IVF in Nepal having a modern lab and professional doctor to conduct this process.